The present manuscript describes simple, sensitive, rapid, accurate, precise and economical first order derivative spectrophotometry method for the determination of chlorhexidine gluconate in mouthwash. The Absorbance value at 272 nm of first derivative spectrum was used for the estimation of chlorhexidine gluconate using methanol as a solvent. This method was found to be linear (r2 > 0.9982) in the range of 10-70 µg/ml at 272 nm. The limit of detection (LOD) was 0.0733 µg/ml. The limit of quantification (LOQ) was 0.2222 µg/ml. This method was successfully applied for the determination of chlorhexidine gluconate in mouthwash. No interference was observed from excipients present in the mouthwash. The suitability of this method for the quantitative determination of chlorhexidine gluconate was proved by validation. The proposed method was found to be simple and sensitive for the routine analysis of chlorhexidine gluconate in mouthwash. The results of analysis have been validated statistically and by recovery studies.
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